FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4076536
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG92340.22651584580634146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTGC5250.010.5697868
GTATTAG11050.010.1831611
TATACGG9750.09.9984465
GTATTAA12600.09.763981
TATACTG11800.09.6595155
TATAATA26150.09.5783322
GTATTAT18100.09.4495231
ACGGTGT9350.09.3033678
CATATAC18400.09.21052553
CTGTGCG2803.324203E-89.1057279
TCTTATA13350.09.1001562
TATTATA23600.08.9609122
ATAATAC18450.08.9417013
GTATAGG7450.08.8609451
TACGGTG9350.08.8221597
CTGCTCG3101.8597348E-88.7083249
ATACGGT11200.08.7040046
GTATATA27550.08.6588291
GTATAAT15000.08.6018291
CTATACT10150.08.5700974
Produced by FastQC (version 0.11.2)