FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_87.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_87.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4860700
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG91960.18919085728393029No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG6750.011.1098655
GTATTAG11700.010.2586371
TACACCG4900.09.4887315
TATTAGG12150.09.1351552
GTATTAT19550.09.0556751
CTAGGAC2651.388853E-79.0554953
GTATATA26350.08.8823831
GGTAGTC6350.08.7391787
CCTATAC6350.08.7390873
TATACTG15700.08.6932925
CTAATAC12100.08.6766233
TATAATA33800.08.6532322
GCCGCGT2957.7081495E-88.64505525
CTATACT11650.08.6256424
TATTATA27500.08.617572
CGCGCAT2803.221885E-78.57073210
ATTAGGT11650.08.4968143
GTATAAA15750.08.4780311
GTATAGA11650.08.3709151
TAGTCAG7100.08.2387689
Produced by FastQC (version 0.11.2)