FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_86.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_86.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4370837
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG78170.17884446388643638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGCG2651.10958354E-1010.7531669
GTATTAG11500.010.696681
CCCGGGG1150.002073316110.4368627
GTATTAA11900.09.95894051
CTAATAC12700.09.9197163
CCTAATA13450.09.8130472
TTATACT14300.09.6487374
CTATGCA9350.09.6242819
CTAGGAC2651.3560566E-89.6211443
ACTATGC11750.09.5730888
TATTAGG11050.09.5012192
TAATACT18700.09.2230594
TCTATAC9600.09.2173163
TAGGTAG4900.09.1821395
GACGCGC2003.227471E-58.9987038
TGCGCGC2352.4328874E-68.93631420
GTATAGA10600.08.9159511
TATACTG14550.08.8644995
CACTCCG3051.4068064E-88.85199414
TACTATG13400.08.8419087
Produced by FastQC (version 0.11.2)