FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1805659
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43980.24356758391257705No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCCG706.043205E-414.99869814
CGGCCGA650.00639158413.84495214
CTAGACT900.004011198411.6656544
GTATTAG3850.010.9120721
GGGTACG1000.0087625710.5028711
ACGGTGT2156.4606866E-810.4642088
TAGACAG2803.0013325E-1010.1776885
TATTAGG4500.09.999412
CCTATAG2003.0329156E-69.75266551
CGATCAC1409.707961E-49.64362224
CGACTAA1250.0041461069.59890210
CTAATAC4550.09.559613
AGTCCGG2209.932919E-79.54436210
GACCATC2054.174113E-69.511377
GAAGTGC2403.2129174E-79.37418656
CCTGGAC1603.1498613E-49.37418653
CACCGCG1450.00133940319.31005323
TCCTAGG1300.0057256139.2302242
AGCCGGA2301.8108531E-69.13217230
CCTAGCG1500.00182060199.00171230
Produced by FastQC (version 0.11.2)