FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2955313
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46230.15643013108932963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG4950.012.1196545
TAGTCAG4250.011.6457589
GGTAGTC4350.011.0330647
TATTAGG9050.010.938192
GTATTAG9250.010.705131
CGCGGCA1501.6431649E-410.00108326
AGTCAGA5450.09.90715110
CCCGTCA1801.01043785E-49.16657414
GTATAGA8200.09.1484161
ATTAGGT8100.09.0730613
TAGACTA2157.653774E-69.0686015
TTAGGTA7950.09.0554394
TATAATA30900.08.9797322
CTAATAC8400.08.9275753
TAATACT13550.08.854954
TCGCCCG1705.768769E-48.82418624
CTATGCA5750.08.6077359
CTATACT8400.08.5703274
TATACTG10850.08.5703265
TAGGGTG3357.688868E-98.506375
Produced by FastQC (version 0.11.2)