FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_83.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_83.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3438641
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63240.18390986439119408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG4200.013.2129855
GTATTAG8900.011.8007111
GGTAGTC4850.010.514437
CTATGCA6950.010.1428869
CGTGCAC1501.646774E-49.99901610
TAGTCAG4050.09.9990169
ACTATGC9800.09.9488
TAATACT17750.09.6328554
TATACTG13200.09.430895
GCGCACT2557.678864E-89.41111311
CTAATAC11750.09.3182333
TTATACT14150.09.2217064
TCACACA6700.09.1804730
TGTGCGC1801.0115383E-49.16576510
ATCACAC6600.09.09226329
TTAGGTA8450.09.0523634
GTCTTAG4150.09.0384091
GGGGCTC2352.4360234E-68.9352910
GTATTAA11800.08.9005361
CCTAATA10650.08.8726242
Produced by FastQC (version 0.11.2)