FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_82.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_82.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences694725
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19780.2847169743423657No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGAC350.003008737521.4239443
GTATAGA1750.017.1477931
GTTAAGC653.439821E-416.1503583
CTTGGAC1052.4798646E-715.7108943
AGGGTGT851.6349726E-414.1145996
CTAGACA750.001018895713.9969774
CTGGACT750.001018895713.9969774
TAGACAC650.00639183113.8431645
TGTACGA650.00639183113.8431642
GTATTAC1302.7450496E-612.6959651
GTGCAGG1006.4277265E-412.0034561
ACCAGTG1006.455014E-411.9974097
CATACTG1006.455014E-411.9974095
ACCTTGC1006.455014E-411.9974098
CAGTGCA1151.5787907E-411.7374419
CAGTGTC900.004008665711.6649879
CTAATTC1551.544111E-611.6103963
CTATGCA1459.33037E-611.3776729
ACCTAAG950.005979728411.0558151
TTAATAC1501.3603383E-510.9976243
Produced by FastQC (version 0.11.2)