FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1482925
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGCG901.5773441E-515.00484130
GTATTAG4150.014.821061
TTAGGTA2850.014.2079234
GCCCGGT750.001019601113.9974367
TAGTCAG2150.013.9509329
CTAATAC4300.013.9509323
TATTAGG3850.013.2443272
TGCCTCG1151.09285975E-513.04416818
GCAGTCC1151.0955209E-513.0410898
TAGGTAG3150.012.3786855
CCTAATA4150.011.9255262
ACAGCTC1652.5621011E-711.8160168
GGTAGTC2701.8189894E-1211.664537
GTATTAA4050.011.1124481
CTTAGGG950.0060061811.0506073
TATACGG2751.8189894E-1110.9070935
CTACACA1653.2798216E-610.9070924
GTGGCGT1807.843082E-710.83682930
ACAGTGT3500.010.7123238
TAAGGCG2251.0992153E-810.66723118
Produced by FastQC (version 0.11.2)