FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016625
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23520.2313537440059019No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG2700.016.1155241
TAGGTAG1651.2751116E-913.6347325
GGCGCCG800.001655217513.12601225
TATTAGG2400.013.1253662
GGTAGTC1507.388371E-812.9984457
CGCCGAA954.1822201E-412.63317527
TACCCTG954.1909062E-412.6300695
CTATAAG1456.8701775E-712.4171931
GTGTTAA1702.8136128E-812.3563241
CCCCCGC850.002611356812.35328621
CTAATAC2550.012.3514633
CTAGGTA850.00261424912.3514624
GCGCCGA1101.041919E-412.27367326
AGTCTAT900.003998349411.6698631
TAGGACA900.004010124611.665274
TAAACTG1551.5446458E-611.6115135
GACCGTC1303.8471713E-511.537087
TCTACAC1703.7652353E-711.4692153
CTGGCGC1059.7108365E-411.42832823
CTATACA2259.113137E-1011.3319764
Produced by FastQC (version 0.11.2)