FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2810981
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG61840.2199943720715295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG4050.016.665575
CTAATAC8800.014.3174953
GGACCGC650.00639186513.8452426
CCTAGCG2750.013.637650530
GTATTAG6850.013.1420291
ACTATGC8550.012.2798948
CGATCAC3250.012.00091724
AATACTA9950.011.9087645
TAATACT13000.011.76845554
TATTAGG7100.011.6191612
CTAGGCA3400.011.46983310
TCTATAC8700.011.3787633
GTATAGA8500.011.2969911
TTAGGTA6400.011.2492594
GTCCTAA3900.011.1566851
GTTCTAA7150.011.121711
ACCTAGC3250.011.078361529
GGTAGTC4500.010.9992767
ATCACAC6300.010.95380329
CCTAATA8200.010.7921652
Produced by FastQC (version 0.11.2)