FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9266975
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG166250.17940050555871792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATAC26100.010.5158543
TAATACT41550.010.2512444
GTATTAG24200.010.2292351
CCGTGCG2701.8402716E-89.4431989
CGGTCGG3502.0008883E-119.4280913
GTATTAT43000.09.385521
GTATAGA26650.09.2888361
TATACAG37300.09.2479585
ACTATGC24550.09.1026688
CTATGCA20350.08.9914429
TAGGTAG11750.08.9348465
GTATATA58150.08.9269051
CCTAATA26150.08.8899842
GTATTAA27250.08.8640851
GTATAAT36350.08.6674121
TATTAGG22250.08.6282142
TAGACTG9450.08.5701585
TAGGCCG3152.444358E-88.5701585
TATTATA59450.08.5271872
TATACGG18300.08.5233275
Produced by FastQC (version 0.11.2)