FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_77.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_77.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6441027
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG81960.12724678843917284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG19150.010.7323261
GGTAGTC9500.010.4199257
TAGTCAG9500.010.104179
CTAATAC20800.09.8789693
TAGGTAG9900.09.6958465
CCTAGCG4350.09.65697830
GTATTAA23150.09.0723351
GACCGTG3001.0621079E-88.9990277
TAATACT34200.08.858764
TATACTG24100.08.7749175
TACGGTG12550.08.7241557
CCTAATA22700.08.6559222
GTATAGA18650.08.6069051
ACGGTGT12200.08.6056268
CTATGCA15150.08.513939
ACTATGC17100.08.5078528
GTATTAT35300.08.49957751
TAGACTG6950.08.4162915
GACATAG10900.08.39606430
TACACTG10100.08.3158675
Produced by FastQC (version 0.11.2)