FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1783646
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31840.17851075830069418No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG2001.2914825E-1012.7486185
GCCGCGT954.1823683E-412.63410625
CGCCTAA1006.457924E-411.99869912
CTAACAC2301.05501385E-1011.7381873
CCAGGAC1951.5344995E-811.5375343
GTATTAG4950.011.519071
GTCCAGA1602.253797E-611.2538291
GACAACG2251.1026714E-810.6655097
CAGGTCG1000.00878732610.4988629
GCTCGGA1000.00878732610.4988624
AGGACTG2053.7798418E-710.2427925
GGTAGTC2505.2059477E-910.19889457
GTGCGAC2251.2509736E-710.00229
CGAGAAC1356.966076E-49.99891714
CGTCCTC1200.00295787899.99891610
GTCATAG2157.2126204E-79.7707661
GGACAAC2651.3520548E-89.6215986
GTCTAGG2054.1513704E-69.5154321
CACGCAG2054.1736675E-69.51143116
GTCTATG2854.3582986E-99.4769091
Produced by FastQC (version 0.11.2)