FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_75.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_75.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12350214
Sequences flagged as poor quality0
Sequence length36
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG189120.15313094979568775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA77300.09.4326351
TATACTG34950.08.9261845
CGCGCGA1550.0024604978.70894810
CCGTCCC2957.7387085E-88.6433449
CTTAGAC13400.08.6186833
CCCGCCG1757.7086827E-48.57140523
GTCTAGG7900.08.5459771
GTATAGG19050.08.5056021
CTGGGCC4453.6379788E-128.4262619
ACCGTGC4651.8189894E-128.3863288
TCTATAC33650.08.2013943
CTAAGAC9950.08.1400223
GTATTAG28200.08.1398921
GTCTATA28200.08.1398921
GTATTAT52250.08.0111371
TTATACT48750.07.99926574
GTCTTAG8300.07.9533641
GTATTAA36100.07.9378421
ACCGCTC3602.773595E-87.91594088
CCGCTCC3051.1800294E-67.86819369
Produced by FastQC (version 0.11.2)