FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_74.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_74.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5785703
Sequences flagged as poor quality0
Sequence length36
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG98700.1705929253541013No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGCGC1250.0041496069.5985768
GTATTAA26850.09.0512841
GTATAGG9050.08.7855041
GTATAGA14900.08.5579971
TATACTG16950.08.4943155
TTATACT20300.08.4223954
TCTTATA22650.08.3432052
ACTGCGC1800.00102079318.3320978
GTATATA30600.08.2852331
GTCTAGG3451.29602995E-88.2617861
TATACAG19250.08.1806045
GTATTAT35950.08.1789321
TAGACTG5900.08.1343865
GTCTTAA11750.08.0434461
CTAGTAC6900.08.0422153
GTATAAA21300.08.029061
TAGGGGT4505.0931703E-117.9988134
TAATACT25800.07.96393444
GTACTAA12250.07.96006631
TATAATA54650.07.9586312
Produced by FastQC (version 0.11.2)