FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2838522
Sequences flagged as poor quality0
Sequence length36
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG80190.2825061775106904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTATAC4100.010.2426333
CTAGACT4450.010.1112844
TATACCG3800.09.86737255
TCTAGAC5350.09.8118693
TAGACTG3551.8189894E-129.7172785
TAATACT3401.0913936E-119.704854
GTATAGG3101.8553692E-109.679461
GTGTTAT2352.3588473E-79.5764871
CGTTATA2352.3691427E-79.5736192
GTATATA7350.09.3897341
ATATTAT5000.09.3019611
GTTATAC3552.7284841E-119.2946233
TAGGAGT3306.1118044E-109.0899434
ACCGTGC2651.3873978E-79.0556418
GTCCTAC2003.2127675E-59.0018981
TAAACCG2505.807906E-78.9990435
TAGCACC3851.4551915E-118.9600864
GTATAGC2854.401045E-88.9492561
TACTAAT2352.4350738E-68.9353782
TATAATA5050.08.9101012
Produced by FastQC (version 0.11.2)