FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301972
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9900.3278449657584147No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCCT350.002996180421.43460727
ATAAGGC504.5958324E-520.995483
GTTCTGA550.002099836516.3601136
TAAGACA706.02845E-414.9967714
ATACTGG650.006380778313.8431736
AAAAGCT1004.2587333E-513.4970944
TAAATAG1151.574179E-411.7366035
TTATACC1059.6920534E-411.4261114
TTACAAG1100.001429952210.9067423
CTCTAAT1000.00873368210.5029571
TTTATCC1000.008765927510.497743
TATACTT1150.002070845310.4325365
GTATTAT1450.0013288379.3129671
CTAAATA1350.007784538.8869754
AAATTCA1550.00244541448.70924515
TAATACT1850.0013304648.1063634
GTATTAA1700.0056124667.94341331
TATAATT2000.0028598187.49962762
TATTGAA2150.0057871716.97639752
Produced by FastQC (version 0.11.2)