FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2260845
Sequences flagged as poor quality0
Sequence length36
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41330.1828077555073435No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGCT901.5845078E-514.9983854
GTATTAG2750.012.5482661
GTATAAT4800.011.2525221
GTATATA7150.011.1213741
GTCTATA3400.010.590611
CTAGGAC1150.00207794410.433663
TAGGCTG1304.9231463E-410.3834975
GTTCTAA2557.1704562E-910.0022421
TACACCG3900.09.9989245
CCTATAG1653.9012186E-59.9989233
CTTATAC3051.364242E-109.8350073
TATAGCA3750.09.5996042
TAATACT3950.09.4926484
GTCTAGG1757.4084914E-59.4306851
GGCTTAG1450.00133655499.3124321
GTCCTAG1300.0057132199.2328391
TAACGTG1300.0057288369.2297755
CCCTTAT1654.2744778E-49.0929481
TCTAAGC2157.648827E-69.0687913
TCAATAC5150.09.0281543
Produced by FastQC (version 0.11.2)