##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	HK5NHBGXX_combined_n02_ntr_6.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27
Sequences flagged as poor quality	0
Sequence length	36
%GC	38
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0	0.0	0.0	0.0	0.0	0.0
2	32.0	0.0	0.0	0.0	0.0	0.0
3	31.333333333333332	0.0	0.0	0.0	0.0	0.0
4	31.14814814814815	0.0	0.0	0.0	0.0	0.0
5	29.333333333333332	0.0	0.0	0.0	0.0	0.0
6	35.333333333333336	0.0	0.0	0.0	0.0	0.0
7	34.03703703703704	0.0	0.0	0.0	0.0	0.0
8	34.888888888888886	0.0	0.0	0.0	0.0	0.0
9	34.51851851851852	0.0	0.0	0.0	0.0	0.0
10	35.03703703703704	0.0	0.0	0.0	0.0	0.0
11	35.03703703703704	0.0	0.0	0.0	0.0	0.0
12	36.0	0.0	0.0	0.0	0.0	0.0
13	34.925925925925924	0.0	0.0	0.0	0.0	0.0
14	33.111111111111114	0.0	0.0	0.0	0.0	0.0
15	35.03703703703704	0.0	0.0	0.0	0.0	0.0
16	36.0	0.0	0.0	0.0	0.0	0.0
17	35.03703703703704	0.0	0.0	0.0	0.0	0.0
18	35.851851851851855	0.0	0.0	0.0	0.0	0.0
19	34.888888888888886	0.0	0.0	0.0	0.0	0.0
20	33.18518518518518	0.0	0.0	0.0	0.0	0.0
21	33.55555555555556	0.0	0.0	0.0	0.0	0.0
22	33.407407407407405	0.0	0.0	0.0	0.0	0.0
23	34.370370370370374	0.0	0.0	0.0	0.0	0.0
24	34.592592592592595	0.0	0.0	0.0	0.0	0.0
25	35.55555555555556	0.0	0.0	0.0	0.0	0.0
26	35.51851851851852	0.0	0.0	0.0	0.0	0.0
27	34.333333333333336	0.0	0.0	0.0	0.0	0.0
28	35.148148148148145	0.0	0.0	0.0	0.0	0.0
29	35.851851851851855	0.0	0.0	0.0	0.0	0.0
30	33.629629629629626	0.0	0.0	0.0	0.0	0.0
31	33.25925925925926	0.0	0.0	0.0	0.0	0.0
32	34.2962962962963	0.0	0.0	0.0	0.0	0.0
33	34.407407407407405	0.0	0.0	0.0	0.0	0.0
34	34.074074074074076	0.0	0.0	0.0	0.0	0.0
35	33.888888888888886	0.0	0.0	0.0	0.0	0.0
36	35.51851851851852	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
21610	1	0.0
21610	2	0.0
21610	3	9.0
21610	4	9.0
21610	5	9.0
21610	6	0.0
21610	7	11.0
21610	8	9.0
21610	9	4.5
21610	10	4.5
21610	11	0.0
21610	12	0.0
21610	13	0.0
21610	14	0.0
21610	15	11.0
21610	16	0.0
21610	17	-11.0
21610	18	0.0
21610	19	11.0
21610	20	11.0
21610	21	0.0
21610	22	11.0
21610	23	11.0
21610	24	-9.0
21610	25	2.0
21610	26	2.0
21610	27	2.0
21610	28	0.0
21610	29	0.0
21610	30	0.0
21610	31	4.5
21610	32	11.0
21610	33	0.0
21610	34	0.0
21610	35	11.0
21610	36	4.5
21308	1	0.0
21308	2	0.0
21308	3	-9.0
21308	4	-9.0
21308	5	-9.0
21308	6	0.0
21308	7	-11.0
21308	8	-9.0
21308	9	-4.5
21308	10	-4.5
21308	11	0.0
21308	12	0.0
21308	13	0.0
21308	14	0.0
21308	15	-11.0
21308	16	0.0
21308	17	11.0
21308	18	0.0
21308	19	-11.0
21308	20	-11.0
21308	21	0.0
21308	22	-11.0
21308	23	-11.0
21308	24	9.0
21308	25	-2.0
21308	26	-2.0
21308	27	-2.0
21308	28	0.0
21308	29	0.0
21308	30	0.0
21308	31	-4.5
21308	32	-11.0
21308	33	0.0
21308	34	0.0
21308	35	-11.0
21308	36	-4.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
26	1.0
27	0.0
28	0.0
29	0.0
30	0.0
31	1.0
32	3.0
33	3.0
34	9.0
35	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.629629629629626	29.629629629629626	25.925925925925924	14.814814814814813
2	11.11111111111111	18.51851851851852	44.44444444444444	25.925925925925924
3	11.11111111111111	25.925925925925924	37.03703703703704	25.925925925925924
4	14.814814814814813	25.925925925925924	44.44444444444444	14.814814814814813
5	11.11111111111111	33.33333333333333	51.85185185185185	3.7037037037037033
6	22.22222222222222	37.03703703703704	29.629629629629626	11.11111111111111
7	18.51851851851852	25.925925925925924	44.44444444444444	11.11111111111111
8	22.22222222222222	29.629629629629626	22.22222222222222	25.925925925925924
9	44.44444444444444	18.51851851851852	11.11111111111111	25.925925925925924
10	11.11111111111111	22.22222222222222	40.74074074074074	25.925925925925924
11	14.814814814814813	48.148148148148145	22.22222222222222	14.814814814814813
12	14.814814814814813	25.925925925925924	44.44444444444444	14.814814814814813
13	14.814814814814813	22.22222222222222	25.925925925925924	37.03703703703704
14	22.22222222222222	22.22222222222222	40.74074074074074	14.814814814814813
15	22.22222222222222	29.629629629629626	25.925925925925924	22.22222222222222
16	11.11111111111111	22.22222222222222	40.74074074074074	25.925925925925924
17	33.33333333333333	22.22222222222222	22.22222222222222	22.22222222222222
18	18.51851851851852	40.74074074074074	25.925925925925924	14.814814814814813
19	14.814814814814813	44.44444444444444	29.629629629629626	11.11111111111111
20	18.51851851851852	33.33333333333333	37.03703703703704	11.11111111111111
21	25.925925925925924	33.33333333333333	29.629629629629626	11.11111111111111
22	22.22222222222222	29.629629629629626	25.925925925925924	22.22222222222222
23	25.925925925925924	29.629629629629626	29.629629629629626	14.814814814814813
24	18.51851851851852	22.22222222222222	51.85185185185185	7.4074074074074066
25	11.11111111111111	18.51851851851852	44.44444444444444	25.925925925925924
26	18.51851851851852	33.33333333333333	25.925925925925924	22.22222222222222
27	22.22222222222222	22.22222222222222	37.03703703703704	18.51851851851852
28	22.22222222222222	33.33333333333333	14.814814814814813	29.629629629629626
29	11.11111111111111	40.74074074074074	25.925925925925924	22.22222222222222
30	14.814814814814813	37.03703703703704	25.925925925925924	22.22222222222222
31	14.814814814814813	44.44444444444444	29.629629629629626	11.11111111111111
32	22.22222222222222	25.925925925925924	29.629629629629626	22.22222222222222
33	25.925925925925924	29.629629629629626	29.629629629629626	14.814814814814813
34	14.814814814814813	40.74074074074074	25.925925925925924	18.51851851851852
35	18.51851851851852	33.33333333333333	29.629629629629626	18.51851851851852
36	25.925925925925924	14.814814814814813	37.03703703703704	22.22222222222222
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.5
22	1.0
23	1.0
24	3.0
25	5.0
26	2.5
27	0.0
28	0.0
29	1.5
30	3.0
31	3.0
32	2.0
33	1.0
34	1.0
35	1.5
36	2.0
37	2.0
38	2.0
39	2.0
40	3.5
41	5.0
42	5.0
43	3.5
44	2.0
45	2.0
46	2.0
47	2.0
48	2.0
49	1.5
50	1.0
51	1.5
52	2.0
53	2.0
54	1.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.5
61	1.0
62	1.0
63	0.5
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
36	27.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	100.0
#Duplication Level	Percentage of deduplicated	Percentage of total
1	100.0	100.0
2	0.0	0.0
3	0.0	0.0
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTCAAGCTGCTTTAATAGGTGATTTATTCATCCCGG	1	3.7037037037037033	No Hit
CTGTTAGGTAAAATCGACCTTGTATCAAATAACAAG	1	3.7037037037037033	No Hit
GCCAGTACATTCCCCAGATAAGGCCGTGCGCGAAGC	1	3.7037037037037033	No Hit
ATTGACTATTCTTGTGGTGCTTCTATTACCGGGTTT	1	3.7037037037037033	No Hit
GATCTTTCGCAGCAGATGATTCATCACCACTTAAGT	1	3.7037037037037033	No Hit
TCTTTAGTCAATGAGTGTAGTGGTTTAAATACTTGT	1	3.7037037037037033	No Hit
GGTGATTAGTAATTGCCGAATGATCAGTGGTCAGCT	1	3.7037037037037033	No Hit
CATATATAGCAGCACCTCTAGATTTCTCCTTCCTTC	1	3.7037037037037033	No Hit
ATCTTCTTTCTTGGTCTTGGGCGGGTACTCGAAATG	1	3.7037037037037033	No Hit
AGATCAAACTGAGGCGGAGATAATAGCCGACAGGAT	1	3.7037037037037033	No Hit
ATCCAGAAGTACCTGAGAATAACTCAGCAAATGACG	1	3.7037037037037033	No Hit
GGGGTACCGTGAATACACTTTTCACCTATGAAGGCC	1	3.7037037037037033	No Hit
ATACTGTGGCTTCGTTGAAAGAACAACAACTAGATT	1	3.7037037037037033	No Hit
TCCTTAACGGATCTTTTAAAATTATTTAACTGTTTC	1	3.7037037037037033	No Hit
TAAGTAGTAACATCATCCAAAGATAATCTAATAAAA	1	3.7037037037037033	No Hit
TTTTAGTGCCGGTATATTCTCCATTGGAGTATTGAA	1	3.7037037037037033	No Hit
ACATACTCCTATCTAAAAATCTTTTTGTTATCGAAA	1	3.7037037037037033	No Hit
TCGTTTACCTCTTCCAGATTATTATCAGATGGTAGG	1	3.7037037037037033	No Hit
ACTAGAAGAAAGAGATGTAAGGGTACCGTTGGTAAC	1	3.7037037037037033	No Hit
GACAAAGAGTTTATATGTATCAAGTATGCAATTTAT	1	3.7037037037037033	No Hit
TTATAGTGGAATCATCCGAAACGGGCCCCACTACCG	1	3.7037037037037033	No Hit
GTAATTATGAAACGATCATCATTGGGAAAAAATATT	1	3.7037037037037033	No Hit
GCTTGTTCACACCTGTCATGGTCATGGGATTTTCAT	1	3.7037037037037033	No Hit
TTTATACACTATATATATTCAAGTTTGATAACCCTC	1	3.7037037037037033	No Hit
CACCTGTGAGCAGTCCAAAGAAGGCAAGAGATGCCA	1	3.7037037037037033	No Hit
CTTTTTTACTGCTTGTTGCTTCTTCTTTTAAGATAG	1	3.7037037037037033	No Hit
ATATATGTGGTTACTCCATGGCAATATCACAAATTT	1	3.7037037037037033	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10	0.0	0.0	0.0
11	0.0	0.0	0.0
12	0.0	0.0	0.0
13	0.0	0.0	0.0
14	0.0	0.0	0.0
15	0.0	0.0	0.0
16	0.0	0.0	0.0
17	0.0	0.0	0.0
18	0.0	0.0	0.0
19	0.0	0.0	0.0
20	0.0	0.0	0.0
21	0.0	0.0	0.0
22	0.0	0.0	0.0
23	0.0	0.0	0.0
24	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE