FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27
Sequences flagged as poor quality0
Sequence length36
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAAGCTGCTTTAATAGGTGATTTATTCATCCCGG13.7037037037037033No Hit
CTGTTAGGTAAAATCGACCTTGTATCAAATAACAAG13.7037037037037033No Hit
GCCAGTACATTCCCCAGATAAGGCCGTGCGCGAAGC13.7037037037037033No Hit
ATTGACTATTCTTGTGGTGCTTCTATTACCGGGTTT13.7037037037037033No Hit
GATCTTTCGCAGCAGATGATTCATCACCACTTAAGT13.7037037037037033No Hit
TCTTTAGTCAATGAGTGTAGTGGTTTAAATACTTGT13.7037037037037033No Hit
GGTGATTAGTAATTGCCGAATGATCAGTGGTCAGCT13.7037037037037033No Hit
CATATATAGCAGCACCTCTAGATTTCTCCTTCCTTC13.7037037037037033No Hit
ATCTTCTTTCTTGGTCTTGGGCGGGTACTCGAAATG13.7037037037037033No Hit
AGATCAAACTGAGGCGGAGATAATAGCCGACAGGAT13.7037037037037033No Hit
ATCCAGAAGTACCTGAGAATAACTCAGCAAATGACG13.7037037037037033No Hit
GGGGTACCGTGAATACACTTTTCACCTATGAAGGCC13.7037037037037033No Hit
ATACTGTGGCTTCGTTGAAAGAACAACAACTAGATT13.7037037037037033No Hit
TCCTTAACGGATCTTTTAAAATTATTTAACTGTTTC13.7037037037037033No Hit
TAAGTAGTAACATCATCCAAAGATAATCTAATAAAA13.7037037037037033No Hit
TTTTAGTGCCGGTATATTCTCCATTGGAGTATTGAA13.7037037037037033No Hit
ACATACTCCTATCTAAAAATCTTTTTGTTATCGAAA13.7037037037037033No Hit
TCGTTTACCTCTTCCAGATTATTATCAGATGGTAGG13.7037037037037033No Hit
ACTAGAAGAAAGAGATGTAAGGGTACCGTTGGTAAC13.7037037037037033No Hit
GACAAAGAGTTTATATGTATCAAGTATGCAATTTAT13.7037037037037033No Hit
TTATAGTGGAATCATCCGAAACGGGCCCCACTACCG13.7037037037037033No Hit
GTAATTATGAAACGATCATCATTGGGAAAAAATATT13.7037037037037033No Hit
GCTTGTTCACACCTGTCATGGTCATGGGATTTTCAT13.7037037037037033No Hit
TTTATACACTATATATATTCAAGTTTGATAACCCTC13.7037037037037033No Hit
CACCTGTGAGCAGTCCAAAGAAGGCAAGAGATGCCA13.7037037037037033No Hit
CTTTTTTACTGCTTGTTGCTTCTTCTTTTAAGATAG13.7037037037037033No Hit
ATATATGTGGTTACTCCATGGCAATATCACAAATTT13.7037037037037033No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)