FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_69.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_69.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380000
Sequences flagged as poor quality0
Sequence length36
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAT651.6369753E-518.4670471
CTAAGGC550.00209951516.3620573
GTCTAAG952.6186117E-514.2147671
CATATAC851.6302429E-414.1162853
TCTATAC750.001016485713.9986493
ATTATAC1004.259657E-513.4986973
CTAAGAC902.6467984E-413.33204653
TTGCAGA800.001654060113.123733511
GGATAAG954.16547E-412.6353481
TTATACC954.1793706E-412.6303614
TCTAAGG850.00260602712.3533752
TACAGAT850.00260602712.3533752
TAAGGCA1101.0402939E-412.27154354
CTCCTGA900.003989745411.6701481
CTCTATA900.003997602511.6670752
AAGAGTA900.00400153611.6655415
TAGACAG900.00400153611.6655415
TCTAACC900.00400153611.6655413
ATACAGT1059.6925005E-411.4274686
ACACCGA950.005988398611.0515657
Produced by FastQC (version 0.11.2)