FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3336685
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG53110.159169954610639No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCCA550.002102266316.36422521
ACTGCGC951.5131391E-615.7874388
CGCGCGT706.0470385E-414.9980677
CCGACCG1201.6975217E-512.4985769
GTGTAGA3250.012.0022311
GCGCGTG1006.461636E-411.9984538
GCGACTA1355.6422825E-511.11350929
GCGGCCC950.006004407511.05170414
TAGCGCG950.006007756611.0508774
GCAGCGC1150.002076943610.43453216
GTATAGG4550.010.221681
GTGTATA6350.010.1593691
GTATTAG11750.010.0869811
ACCGTGT2251.2586133E-79.9987128
GACCGTG1356.9707754E-49.9987117
TACACGT1200.0029602689.9984114
GTATAAA15400.09.8394921
GTATATG8550.09.8263871
GTATAAG13950.09.7867651
GTCTAGG2301.72613E-79.7844271
Produced by FastQC (version 0.11.2)