FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_66.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_66.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences359670
Sequences flagged as poor quality0
Sequence length36
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17210.4784941752161704No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTGCG400.006506528218.747747
GGTAGTT550.00209955716.3616667
CCTTATT851.6301917E-414.1159462
ACAGAAC650.006379898613.8444858
CTTAGGA650.006379898613.8444852
AGCAGTC650.006379898613.8444857
ATAAGAC800.001654058813.1234183
AACAGCC800.001654058813.1234187
TATATAG1056.732324E-512.8555935
ATACTGT954.1792504E-412.6300566
TTATACT1006.436517E-411.9985544
TAGTAGT1006.436517E-411.9985544
CACACTG900.004001541511.665265
TATACAG1059.6922397E-411.4271945
ATATAAG1202.3321852E-411.2517731
TATAATA1100.00142997410.9077772
CATATAC1253.4128115E-410.7986983
TCACACT1000.00876523710.4987354
ATATAAA1304.88963E-410.3862511
ATATACA1603.1352422E-49.373874
Produced by FastQC (version 0.11.2)