FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7450838
Sequences flagged as poor quality0
Sequence length36
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG84400.11327584897161903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT4250.010.2342168
TACACCG13900.08.9558325
GTATATA24800.08.8318491
CCGCGTG4105.456968E-128.7796229
GTATTAG11800.08.7723811
CGCGTGA3305.8807927E-98.63551110
TATACCG13150.08.5541735
ACCGCGC4403.6379788E-128.521838
GTATAGG10950.08.2202981
GTCTTAG3851.2714736E-98.1829341
TAAGGCT8450.08.1647654
TATTATA22050.08.162572
CTAGGGC4609.094947E-128.151263
TACACAG8750.08.056245
GTATTAT15700.08.0265711
TAGGACA4955.456968E-127.87790444
TAGTACT11850.07.84722144
GTATAGA13500.07.77883771
GTAATAC10100.07.72190673
TAATACT14650.07.67832044
Produced by FastQC (version 0.11.2)