FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4772844
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG79450.16646259546718895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG11450.012.709041
ACCGCGC1901.3296267E-711.0512588
TAGGTAG6100.010.8183265
GGTAGTC5950.010.3348497
GACCGCG1756.6324756E-610.2844357
GGACCGC1250.00414855739.5988066
GGCTCGG3801.8189894E-129.4748891
TAGTCAG6050.09.4203169
TATACTG17200.09.4174345
TATTAGG13400.09.2902792
TAATACT22750.09.2297184
CTAATAC13900.09.171813
CTCGGAG4300.09.0689253
GTATAAA19950.09.0237041
TCTATAC10700.08.9711293
GTCTTAA7250.08.8976831
TAGACTG4600.08.8032535
CTAGACT4500.08.665684
CTGTGCG2601.0230106E-68.652779
TATACCG8700.08.6196175
Produced by FastQC (version 0.11.2)