FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7275185
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG95090.13070458002098917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG17450.010.6608551
GGCTCGG8000.010.50180151
CTCGGAG9300.09.83809953
TATTAGG17400.09.7408422
GCTCGGA9200.09.6190352
TAGACAG11800.09.1517865
CTAATAC18400.09.0483513
GTCCTAA8650.09.0188881
TAGGTAG9650.09.0147985
TAATACT30050.08.934434
TATACTG21850.08.9237475
CTAGGAC4550.08.9005463
GTATAGA19000.08.8436231
GTCTTAG9050.08.7860381
GTATTAA20150.08.7856261
CTTACAC8050.08.757223
ACCGCGA2752.4481596E-78.7265518
ACTATGC16850.08.63438
TAGAACT18400.08.3145884
TAGGACC7400.08.310184
Produced by FastQC (version 0.11.2)