FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4193643
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70700.1685885040762888No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCC600.003757092914.99942213
CTAATAC12300.012.1934883
TAGGTAG5800.011.3779225
CTATGCA9400.011.0094269
ACCGCGC1807.9012534E-710.8320118
CTAGGAC3250.010.6139633
GACCGCG1851.12421E-610.5392557
TAATACT18850.010.2644
CCTAATA11650.010.1710392
ATCACAC7800.010.00176129
CCGTGCG1200.0029593429.9988999
CGCGCAC1653.9034894E-59.998898510
ACCTAAT11850.09.8759811
ACTATGC13000.09.8064968
CGGCCCG2454.141293E-89.795423514
GTATTAG11200.09.7793251
TCACACA8400.09.64455630
CTTACAC5200.09.5179563
TCTACAC8200.09.5109213
CCTTAAG4600.09.4589781
Produced by FastQC (version 0.11.2)