FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5866734
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG100620.1715093951762599No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG17050.013.1114871
TAGGTAG8300.012.6492765
TCCTAGG3001.0004442E-109.9995482
TAATACT26250.09.9990374
TTAGGTA12750.09.8814024
TATTAGG15850.09.8418272
CGCGGCC2003.0499505E-69.74947613
CCTAGCG3850.09.74181330
CTAATAC17250.09.7381933
GTCGGGG1851.283333E-59.72928915
TAGGCTG3950.09.4926765
GTATAGA17000.09.4433251
CCTAATA16600.09.30680752
GGTAGTC9850.09.2883667
ACCGTGC3254.5838533E-109.2298018
CGATCAC4250.09.17730724
ATACAGG17800.08.9316486
TCGGGGG1851.3624295E-48.91866816
GTATTAT27100.08.9134671
ACTATGC17600.08.8627088
Produced by FastQC (version 0.11.2)