FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2652100
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43500.1640209645186833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAGTC3250.011.5373537
GTATTAG7200.011.251041
TAGGTAG3550.010.9848595
CTAGGAG1807.8944504E-710.8322933
CTCGGAG1704.6881196E-610.5872165
GAGTCCG1150.002077949210.433789
ACTATGC6200.010.40222558
TATACTG10900.010.3201075
CCTAGCG1200.002951925610.00167830
CTCCTAG1501.6432839E-410.0009241
GGCTCGG1501.6464134E-49.999043
TTATACT10150.09.9005264
TACACCG2609.911673E-99.8067495
CCTGGAC1705.414128E-59.7049483
TGGACGG1409.7267586E-49.641935
CCGAGTA1409.7267586E-49.641939
TATTAGG7800.09.4225262
ACCCGTG1500.0018259398.9991357
TAGACTG2854.422327E-88.9465085
CTATGCA6550.08.9304399
Produced by FastQC (version 0.11.2)