##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	HK5NHBGXX_combined_n02_ntr_5.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29
Sequences flagged as poor quality	0
Sequence length	36
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.75862068965517	0.0	0.0	0.0	0.0	0.0
2	31.379310344827587	0.0	0.0	0.0	0.0	0.0
3	31.379310344827587	0.0	0.0	0.0	0.0	0.0
4	31.379310344827587	0.0	0.0	0.0	0.0	0.0
5	31.20689655172414	0.0	0.0	0.0	0.0	0.0
6	34.793103448275865	0.0	0.0	0.0	0.0	0.0
7	35.10344827586207	0.0	0.0	0.0	0.0	0.0
8	34.96551724137931	0.0	0.0	0.0	0.0	0.0
9	34.689655172413794	0.0	0.0	0.0	0.0	0.0
10	35.10344827586207	0.0	0.0	0.0	0.0	0.0
11	34.689655172413794	0.0	0.0	0.0	0.0	0.0
12	34.51724137931034	0.0	0.0	0.0	0.0	0.0
13	34.3448275862069	0.0	0.0	0.0	0.0	0.0
14	34.310344827586206	0.0	0.0	0.0	0.0	0.0
15	33.13793103448276	0.0	0.0	0.0	0.0	0.0
16	35.10344827586207	0.0	0.0	0.0	0.0	0.0
17	33.13793103448276	0.0	0.0	0.0	0.0	0.0
18	35.724137931034484	0.0	0.0	0.0	0.0	0.0
19	35.03448275862069	0.0	0.0	0.0	0.0	0.0
20	35.10344827586207	0.0	0.0	0.0	0.0	0.0
21	34.51724137931034	0.0	0.0	0.0	0.0	0.0
22	33.86206896551724	0.0	0.0	0.0	0.0	0.0
23	33.58620689655172	0.0	0.0	0.0	0.0	0.0
24	34.10344827586207	0.0	0.0	0.0	0.0	0.0
25	33.310344827586206	0.0	0.0	0.0	0.0	0.0
26	33.44827586206897	0.0	0.0	0.0	0.0	0.0
27	35.10344827586207	0.0	0.0	0.0	0.0	0.0
28	33.827586206896555	0.0	0.0	0.0	0.0	0.0
29	34.206896551724135	0.0	0.0	0.0	0.0	0.0
30	34.96551724137931	0.0	0.0	0.0	0.0	0.0
31	35.689655172413794	0.0	0.0	0.0	0.0	0.0
32	34.827586206896555	0.0	0.0	0.0	0.0	0.0
33	33.93103448275862	0.0	0.0	0.0	0.0	0.0
34	34.96551724137931	0.0	0.0	0.0	0.0	0.0
35	33.41379310344828	0.0	0.0	0.0	0.0	0.0
36	32.758620689655174	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
13404	1	0.0
13404	2	0.0
13404	3	0.0
13404	4	0.0
13404	5	0.0
13404	6	0.0
13404	7	0.0
13404	8	2.0
13404	9	2.0
13404	10	0.0
13404	11	0.0
13404	12	0.0
13404	13	2.0
13404	14	2.0
13404	15	11.0
13404	16	0.0
13404	17	0.0
13404	18	0.0
13404	19	2.0
13404	20	0.0
13404	21	0.0
13404	22	0.0
13404	23	0.0
13404	24	2.0
13404	25	0.0
13404	26	11.0
13404	27	0.0
13404	28	0.0
13404	29	0.0
13404	30	2.0
13404	31	0.0
13404	32	0.0
13404	33	0.0
13404	34	0.0
13404	35	0.0
13404	36	0.0
12103	1	0.0
12103	2	0.0
12103	3	0.0
12103	4	0.0
12103	5	0.0
12103	6	0.0
12103	7	0.0
12103	8	-2.0
12103	9	-2.0
12103	10	0.0
12103	11	0.0
12103	12	0.0
12103	13	-2.0
12103	14	-2.0
12103	15	-11.0
12103	16	0.0
12103	17	0.0
12103	18	0.0
12103	19	-2.0
12103	20	0.0
12103	21	0.0
12103	22	0.0
12103	23	0.0
12103	24	-2.0
12103	25	0.0
12103	26	-11.0
12103	27	0.0
12103	28	0.0
12103	29	0.0
12103	30	-2.0
12103	31	0.0
12103	32	0.0
12103	33	0.0
12103	34	0.0
12103	35	0.0
12103	36	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
27	1.0
28	1.0
29	2.0
30	0.0
31	0.0
32	1.0
33	3.0
34	6.0
35	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.827586206896555	24.137931034482758	13.793103448275861	17.24137931034483
2	24.137931034482758	31.03448275862069	27.586206896551722	17.24137931034483
3	27.586206896551722	20.689655172413794	27.586206896551722	24.137931034482758
4	6.896551724137931	31.03448275862069	37.93103448275862	24.137931034482758
5	17.24137931034483	41.37931034482759	31.03448275862069	10.344827586206897
6	31.03448275862069	44.827586206896555	10.344827586206897	13.793103448275861
7	31.03448275862069	27.586206896551722	24.137931034482758	17.24137931034483
8	27.586206896551722	24.137931034482758	13.793103448275861	34.48275862068966
9	17.24137931034483	20.689655172413794	37.93103448275862	24.137931034482758
10	24.137931034482758	20.689655172413794	41.37931034482759	13.793103448275861
11	37.93103448275862	31.03448275862069	20.689655172413794	10.344827586206897
12	24.137931034482758	31.03448275862069	20.689655172413794	24.137931034482758
13	34.48275862068966	17.24137931034483	31.03448275862069	17.24137931034483
14	10.344827586206897	31.03448275862069	27.586206896551722	31.03448275862069
15	27.586206896551722	41.37931034482759	13.793103448275861	17.24137931034483
16	31.03448275862069	24.137931034482758	24.137931034482758	20.689655172413794
17	27.586206896551722	20.689655172413794	24.137931034482758	27.586206896551722
18	20.689655172413794	24.137931034482758	41.37931034482759	13.793103448275861
19	24.137931034482758	17.24137931034483	37.93103448275862	20.689655172413794
20	17.24137931034483	34.48275862068966	24.137931034482758	24.137931034482758
21	17.24137931034483	34.48275862068966	34.48275862068966	13.793103448275861
22	13.793103448275861	24.137931034482758	34.48275862068966	27.586206896551722
23	27.586206896551722	34.48275862068966	20.689655172413794	17.24137931034483
24	17.24137931034483	51.724137931034484	20.689655172413794	10.344827586206897
25	31.03448275862069	27.586206896551722	17.24137931034483	24.137931034482758
26	17.24137931034483	37.93103448275862	34.48275862068966	10.344827586206897
27	41.37931034482759	10.344827586206897	27.586206896551722	20.689655172413794
28	37.93103448275862	6.896551724137931	34.48275862068966	20.689655172413794
29	10.344827586206897	31.03448275862069	37.93103448275862	20.689655172413794
30	13.793103448275861	17.24137931034483	34.48275862068966	34.48275862068966
31	27.586206896551722	31.03448275862069	31.03448275862069	10.344827586206897
32	27.586206896551722	27.586206896551722	17.24137931034483	27.586206896551722
33	17.24137931034483	37.93103448275862	27.586206896551722	17.24137931034483
34	24.137931034482758	20.689655172413794	48.275862068965516	6.896551724137931
35	13.793103448275861	34.48275862068966	27.586206896551722	24.137931034482758
36	13.793103448275861	24.137931034482758	41.37931034482759	20.689655172413794
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.5
25	1.0
26	1.0
27	1.0
28	1.0
29	0.5
30	0.0
31	0.0
32	1.0
33	2.0
34	2.0
35	3.0
36	4.0
37	4.0
38	3.0
39	2.0
40	2.0
41	2.0
42	2.0
43	3.0
44	4.0
45	4.0
46	5.0
47	6.0
48	6.0
49	4.5
50	3.0
51	1.5
52	0.0
53	0.0
54	1.5
55	3.0
56	3.0
57	1.5
58	0.0
59	0.0
60	0.5
61	1.0
62	1.0
63	0.5
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
36	29.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	100.0
#Duplication Level	Percentage of deduplicated	Percentage of total
1	100.0	100.0
2	0.0	0.0
3	0.0	0.0
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACAAGTATTGCTACTTCTCTAGGAACGGCCGTGAT	1	3.4482758620689653	No Hit
TAGTATTATAGCTCATGAATGTGGCTCTCTTGATTG	1	3.4482758620689653	No Hit
GTATAAGCTGGATACCTATTTTCATTGGCTTCGTTT	1	3.4482758620689653	No Hit
TAAGGACGCCATTGCGCAGGAATTGAGTTTACAGAG	1	3.4482758620689653	No Hit
GGGCTCGGAGATGTGTATAAGAGACAGGTTTGAGAA	1	3.4482758620689653	No Hit
ATATTACATAGAGCTGCTGATTCCTTTTATGAGTTC	1	3.4482758620689653	No Hit
CGCCAACTTTACAAACTTCGGTTTGACTTTACCGCT	1	3.4482758620689653	No Hit
AGGTTGGGATCCAAACCTTCAACAATTTTGGAATAA	1	3.4482758620689653	No Hit
ATACACGCTCATCAGACACCACAAAAGGTGTTAGTT	1	3.4482758620689653	No Hit
CATTGAACTTTCTTAAAATACTTTGAGCTCTATTAT	1	3.4482758620689653	No Hit
GGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGC	1	3.4482758620689653	No Hit
GCCTTGCCCCTTATGGTGACGAAAGACGGTTGTGAG	1	3.4482758620689653	No Hit
GGTACTTCCATTGCAGACCATAGAAAGGAGGCAATT	1	3.4482758620689653	No Hit
CTCCAGATTGTATCGAGTCCAGCAAGGCCCGAATAT	1	3.4482758620689653	No Hit
GAGAGATACTGGCTGGCGTTAAACGTTAAACCTAAT	1	3.4482758620689653	No Hit
CACTAATGATGGGCAACATCTCAACCGGTCACCGTA	1	3.4482758620689653	No Hit
GATAGAATCTATGTGTTCATTCGAATTACTTTTTAC	1	3.4482758620689653	No Hit
TGGCAGTCATACTCCAATTAAGTGCTACAAGAAAGA	1	3.4482758620689653	No Hit
GCTAACAATAAACAACGTCAACAAAAGCAAAAGCGT	1	3.4482758620689653	No Hit
TCTTAGAGGGACTATCGGTTTCAAGCCGATGGAAGT	1	3.4482758620689653	No Hit
GCGATGACGTGGTCCTTCTTCCATTGGGCGAAGTTG	1	3.4482758620689653	No Hit
ATATAGGCGCTGGGACTTCATGGGTGTGTAGAATCA	1	3.4482758620689653	No Hit
GTTACAGTGGGAGAAACGTGTCAGCTTTTCTGCTTC	1	3.4482758620689653	No Hit
AGATGTGAGAGAGTGTGTGGGTATATATATGTCACT	1	3.4482758620689653	No Hit
GTTCAGGATTAGCTGAGGTACTTAGTGTGCCGTTCC	1	3.4482758620689653	No Hit
GCGTTACGTGGACCAGCTGAATGAGGACCCACATAC	1	3.4482758620689653	No Hit
AACATATCAGCAGTATATAGAGTTCATTACAGGTCA	1	3.4482758620689653	No Hit
GAGCTAGGATTAGCAGGTGTATCATCTGACTTCACT	1	3.4482758620689653	No Hit
CTTGTAAGCTCGAATGAGGATCAACAGCTCACTCCA	1	3.4482758620689653	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10	0.0	0.0	0.0
11	0.0	0.0	0.0
12	0.0	0.0	0.0
13	0.0	0.0	0.0
14	0.0	0.0	0.0
15	0.0	0.0	0.0
16	0.0	0.0	0.0
17	0.0	0.0	0.0
18	0.0	0.0	0.0
19	0.0	0.0	0.0
20	0.0	0.0	0.0
21	0.0	0.0	0.0
22	0.0	0.0	0.0
23	0.0	0.0	0.0
24	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE