FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29
Sequences flagged as poor quality0
Sequence length36
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAAGTATTGCTACTTCTCTAGGAACGGCCGTGAT13.4482758620689653No Hit
TAGTATTATAGCTCATGAATGTGGCTCTCTTGATTG13.4482758620689653No Hit
GTATAAGCTGGATACCTATTTTCATTGGCTTCGTTT13.4482758620689653No Hit
TAAGGACGCCATTGCGCAGGAATTGAGTTTACAGAG13.4482758620689653No Hit
GGGCTCGGAGATGTGTATAAGAGACAGGTTTGAGAA13.4482758620689653No Hit
ATATTACATAGAGCTGCTGATTCCTTTTATGAGTTC13.4482758620689653No Hit
CGCCAACTTTACAAACTTCGGTTTGACTTTACCGCT13.4482758620689653No Hit
AGGTTGGGATCCAAACCTTCAACAATTTTGGAATAA13.4482758620689653No Hit
ATACACGCTCATCAGACACCACAAAAGGTGTTAGTT13.4482758620689653No Hit
CATTGAACTTTCTTAAAATACTTTGAGCTCTATTAT13.4482758620689653No Hit
GGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGC13.4482758620689653No Hit
GCCTTGCCCCTTATGGTGACGAAAGACGGTTGTGAG13.4482758620689653No Hit
GGTACTTCCATTGCAGACCATAGAAAGGAGGCAATT13.4482758620689653No Hit
CTCCAGATTGTATCGAGTCCAGCAAGGCCCGAATAT13.4482758620689653No Hit
GAGAGATACTGGCTGGCGTTAAACGTTAAACCTAAT13.4482758620689653No Hit
CACTAATGATGGGCAACATCTCAACCGGTCACCGTA13.4482758620689653No Hit
GATAGAATCTATGTGTTCATTCGAATTACTTTTTAC13.4482758620689653No Hit
TGGCAGTCATACTCCAATTAAGTGCTACAAGAAAGA13.4482758620689653No Hit
GCTAACAATAAACAACGTCAACAAAAGCAAAAGCGT13.4482758620689653No Hit
TCTTAGAGGGACTATCGGTTTCAAGCCGATGGAAGT13.4482758620689653No Hit
GCGATGACGTGGTCCTTCTTCCATTGGGCGAAGTTG13.4482758620689653No Hit
ATATAGGCGCTGGGACTTCATGGGTGTGTAGAATCA13.4482758620689653No Hit
GTTACAGTGGGAGAAACGTGTCAGCTTTTCTGCTTC13.4482758620689653No Hit
AGATGTGAGAGAGTGTGTGGGTATATATATGTCACT13.4482758620689653No Hit
GTTCAGGATTAGCTGAGGTACTTAGTGTGCCGTTCC13.4482758620689653No Hit
GCGTTACGTGGACCAGCTGAATGAGGACCCACATAC13.4482758620689653No Hit
AACATATCAGCAGTATATAGAGTTCATTACAGGTCA13.4482758620689653No Hit
GAGCTAGGATTAGCAGGTGTATCATCTGACTTCACT13.4482758620689653No Hit
CTTGTAAGCTCGAATGAGGATCAACAGCTCACTCCA13.4482758620689653No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)