FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_59.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_59.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4520214
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55450.12267118326698692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG11800.011.9504441
CTATACT10900.011.4207254
TCTATAC9400.011.0095153
ACTATGC11200.010.98098
CTATGCA9500.010.8936239
CTAATAC12300.010.7306123
TAGGTAG6450.010.6964635
TATTAGG10650.010.5625352
CTAGACT3450.010.4336014
GGTAGTC5800.010.3436577
TAATACT18050.010.0542644
GTCCTAG3451.8189894E-1210.00108051
CCTAATA12550.09.8000132
CTACGCG1250.00414772479.599029
TACTATG13950.09.4612947
CAGCGTG4700.09.25437459
AGTGTGC3901.8189894E-129.2297248
TACACAG6700.09.1780665
CGGTCGC1654.2896127E-49.09018211
CCTATAC8600.09.0688423
Produced by FastQC (version 0.11.2)