FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_58.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_58.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2864957
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54700.19092782195334868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG7200.012.5027361
CCGACGT950.005993659611.05427729
CTATACT8350.010.4179864
TAATACT12600.010.3559734
TAGGTAG4350.010.3436595
GTATATA25250.010.1012191
CTGCCCG1200.00295806639.9992219
CCTAGAC2701.7298589E-99.9990453
CCTATAC5850.09.9990453
TATACTG12300.09.5111215
GTATAGA8450.09.2327891
GTATTAC8150.09.2044681
GTATTAT14050.09.1835041
GTATAAT12850.09.1070511
TAGACTG3152.5465852E-99.04659755
TAGGACT2505.8085607E-78.9989844
TAAGACT4550.08.9000944
CGACGCC1350.0078037998.88897123
ACTATGC7100.08.8723948
TCTATAC9300.08.8701213
Produced by FastQC (version 0.11.2)