FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2043778
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCCG809.760913E-514.99948614
CGATCAC1851.8189894E-1214.5958824
TCGCCCG650.006384564613.84737424
CCTAGCG1855.0567905E-1012.97570330
TCGGTCG1302.7639435E-612.6912529
CTAGGCA2154.292815E-1011.85947910
CGGTCGA1151.5807746E-411.73815310
CACGCAG2701.8189894E-1211.66598116
GTCCTAA2351.546141E-1011.4892491
TTAGGTA3800.011.4458564
CTTACAC3300.011.3618563
GCCTCAC2002.2271706E-811.25044120
CTAGACT2801.8189894E-1211.2485134
TCCTAAG3200.011.2482392
CAGATCC2301.3151293E-911.0860349
CCCCTAG950.00599974811.0525241
GAGCGAC950.00599974811.05252411
TCGCCGA1100.001433334410.90925119
ACTATGC6900.010.8683958
GTATTAG5800.010.8619621
Produced by FastQC (version 0.11.2)