FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_56.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_56.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3069136
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG58080.18923892587360092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG4350.015.5156655
TTAGGTA6400.012.8895264
GTATTAG9200.012.5588021
TAGGCTG1754.247704E-811.9987825
GGTAGTC4800.011.5613267
TACAGCG4700.011.4881947
TAGTCAG4700.011.1692619
TATTAGG7950.010.9428192
CTAATAC8450.010.649983
TCTATAC9600.010.4678573
CCTAATA7600.010.4599752
GTATAGA9850.010.3590031
GTATTAT14250.010.0035481
AGTCAGA6500.09.92223110
TATAATA17800.09.8590322
TATACTG11450.09.8243295
TAATACT14050.09.8212274
ATTAGGT7500.09.7991653
TATACAG13100.09.7318355
GACATAG5750.09.65386730
Produced by FastQC (version 0.11.2)