FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4176387
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG67130.1607370198211995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG11250.013.7352191
TAGGTAG7200.012.706735
GGTAGTC6750.012.4430257
GGCGCCG2600.012.11646725
CTAATAC13750.011.9986313
TAGTCAG6550.011.4490759
CCTAGCG3550.011.41001830
TAATACT20600.011.285124
TTAGGTA10250.011.2670064
TATTAGG11750.011.1053822
ACCGCGC1653.2817643E-610.90784558
TAGGACT3300.010.90784554
TCGCCCG1951.897788E-710.76993624
ATTAGGT10700.010.6529893
CGCGTGC1150.002078690810.4335919
ACCGTGC2609.0039975E-1010.383438
CTATGCA10700.010.2324779
ACTCCCG2557.203198E-910.00029520
TATACTG16050.09.7184015
CGCGCAC1705.4157885E-59.70500710
Produced by FastQC (version 0.11.2)