FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5364808
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG87660.16339820549029901No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG13200.012.6165841
TAGGTAG7950.011.5084765
TAGTCAG7300.010.6841429
TATTAGG13950.010.322012
TAGGACA7050.09.9991674
TATACTG18200.09.8892865
GGTAGTC8150.09.753797
ACCGTGC3700.09.728928
GTATTAA14700.09.6961431
CTGTGCG2251.350285E-69.3326439
GTATATA35300.09.0106041
TAGACTG6500.08.9992515
TCTAGAC5950.08.8227943
CTATACT14900.08.757664
TCTATAC12900.08.7202033
CTAGACA6100.08.605844
GTATAGG11350.08.5923171
TAATACT23250.08.579934
GTATAGA14900.08.4583521
GTCTAAG7100.08.452681
Produced by FastQC (version 0.11.2)