FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1968678
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATATATATACATGAGAAGAACGGCATAGTGCG32920.16721881384360468No Hit
TATATATACAGGCAACACGCAGATATAGGTGCGACG28090.14268458325840996No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26790.13608116715887514No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCC952.6397345E-514.20897413
TGCGACG6250.014.16384930
GTGCGAC6000.013.75408729
CTAATAC7200.013.5391563
CTGTGCG902.657045E-413.33129
GCCGCGT1251.7728344E-613.20358925
GGTGCGA6400.012.89413128
GTATTAG5300.012.7370461
TAGGTAG2600.012.6899555
CAGCTCG2307.2759576E-1212.38932110
GCGGCCG850.002615046712.35190614
CAGCGTG1951.1696102E-912.30572210
CGGCCGA1006.454359E-411.99960415
TGCAGTC3250.011.9977757
ATCACAC4350.011.72762429
CCGCCGT900.004002963711.66894924
GTCCTAC900.00400600411.6677641
GTGGCGT1459.311509E-611.38240430
CGCCGCG1459.319401E-611.381536524
AGGTGCG7500.011.20304627
Produced by FastQC (version 0.11.2)