FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3740031
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG62770.16783283347116643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG9850.011.879821
TAGGTAG4650.010.6436485
TATACTG16500.09.9985775
TATTAGG11300.09.8218752
CGGCCCG1409.7304437E-49.6417449
TGCGACG3451.4551915E-119.56654430
TCGACAC2209.914456E-79.54652226
TATACGG10900.09.3564685
TATACCG7700.09.1545435
TACACCG2957.963536E-99.1512415
TCTATAC11200.09.1058483
GTATAGA11000.09.0012481
GCCGCGT1851.360006E-48.91991725
CCTATAC7950.08.8666643
CTAATAC10050.08.8047183
GTCTAGA3254.449248E-98.7704471
GTCTAGT4650.08.7108851
CCTAGCG1901.8160873E-48.68541430
AGCGCAC2251.3598463E-58.66566710
CTTAGAC4850.08.6585633
Produced by FastQC (version 0.11.2)