FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_51.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_51.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6225183
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG95290.15307180527865608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACT29100.010.4109274
CCTAGCG4400.010.2291430
GTATAGA21550.010.0249521
CTAATAC18300.09.9986943
TAGGTAG7850.09.7438725
ACTATGC14950.09.6307718
AGCGCAC4050.09.62844910
GTATTAG16950.09.470681
TAGACGG4500.09.3320395
GTGCGCG1801.0122459E-49.1654699
GTATATA55650.09.0581861
TAGACAG11500.08.9987525
GTATTAA20250.08.9645281
CTAGTAC9450.08.8877283
TCTATAC17250.08.8684073
GTCTTAG6600.08.8651841
CGATCAC5150.08.73896924
GTATAAT29400.08.6239551
GTACTAG9750.08.6168891
ACCTAGC5600.08.57313229
Produced by FastQC (version 0.11.2)