FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3623476
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60990.16831903950792002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCACT2453.2014214E-1011.01907911
GTATTAG8800.010.5698251
TAGGTAG5550.010.2687495
CTAATAC11900.010.2085713
TATACTG15900.010.0928455
TACACAG7300.010.0670025
TAGTCAG4350.09.9987959
GTCCTAA4300.09.7689611
ACGGCTC1250.00414849039.5987118
ACAGTGC4400.09.5441738
CTATGCA8500.09.5282639
CTTACAC5000.09.2986233
CCTAGCG2454.3186628E-79.18560930
GTCTAGA5250.09.14427951
GGTAGTC5100.09.1164227
CAGCGCA3654.7293724E-119.0400079
CCCGCCG2003.2213837E-59.00003223
GTATTAA10900.08.9463451
TATTAGG9400.08.935342
CTAGGAC2352.4378169E-68.9348473
Produced by FastQC (version 0.11.2)