##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename HK5NHBGXX_combined_n02_ntr_4.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11 Sequences flagged as poor quality 0 Sequence length 36 %GC 34 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.363636363636363 0.0 0.0 0.0 0.0 0.0 2 32.0 0.0 0.0 0.0 0.0 0.0 3 30.363636363636363 0.0 0.0 0.0 0.0 0.0 4 32.0 0.0 0.0 0.0 0.0 0.0 5 32.0 0.0 0.0 0.0 0.0 0.0 6 35.63636363636363 0.0 0.0 0.0 0.0 0.0 7 35.63636363636363 0.0 0.0 0.0 0.0 0.0 8 31.636363636363637 0.0 0.0 0.0 0.0 0.0 9 33.27272727272727 0.0 0.0 0.0 0.0 0.0 10 35.27272727272727 0.0 0.0 0.0 0.0 0.0 11 35.63636363636363 0.0 0.0 0.0 0.0 0.0 12 32.27272727272727 0.0 0.0 0.0 0.0 0.0 13 36.0 0.0 0.0 0.0 0.0 0.0 14 31.272727272727273 0.0 0.0 0.0 0.0 0.0 15 36.0 0.0 0.0 0.0 0.0 0.0 16 36.0 0.0 0.0 0.0 0.0 0.0 17 36.0 0.0 0.0 0.0 0.0 0.0 18 35.63636363636363 0.0 0.0 0.0 0.0 0.0 19 34.81818181818182 0.0 0.0 0.0 0.0 0.0 20 34.0 0.0 0.0 0.0 0.0 0.0 21 36.0 0.0 0.0 0.0 0.0 0.0 22 34.90909090909091 0.0 0.0 0.0 0.0 0.0 23 33.63636363636363 0.0 0.0 0.0 0.0 0.0 24 34.54545454545455 0.0 0.0 0.0 0.0 0.0 25 35.63636363636363 0.0 0.0 0.0 0.0 0.0 26 31.636363636363637 0.0 0.0 0.0 0.0 0.0 27 34.90909090909091 0.0 0.0 0.0 0.0 0.0 28 34.45454545454545 0.0 0.0 0.0 0.0 0.0 29 31.272727272727273 0.0 0.0 0.0 0.0 0.0 30 34.81818181818182 0.0 0.0 0.0 0.0 0.0 31 33.81818181818182 0.0 0.0 0.0 0.0 0.0 32 33.27272727272727 0.0 0.0 0.0 0.0 0.0 33 35.63636363636363 0.0 0.0 0.0 0.0 0.0 34 32.81818181818182 0.0 0.0 0.0 0.0 0.0 35 35.18181818181818 0.0 0.0 0.0 0.0 0.0 36 31.272727272727273 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 12409 1 0.0 12409 2 0.0 12409 3 0.0 12409 4 0.0 12409 5 0.0 12409 6 0.0 12409 7 0.0 12409 8 0.0 12409 9 0.0 12409 10 0.0 12409 11 0.0 12409 12 0.0 12409 13 0.0 12409 14 0.0 12409 15 0.0 12409 16 0.0 12409 17 0.0 12409 18 0.0 12409 19 0.0 12409 20 0.0 12409 21 0.0 12409 22 0.0 12409 23 0.0 12409 24 0.0 12409 25 0.0 12409 26 0.0 12409 27 0.0 12409 28 0.0 12409 29 0.0 12409 30 0.0 12409 31 0.0 12409 32 0.0 12409 33 0.0 12409 34 0.0 12409 35 0.0 12409 36 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 32 4.0 33 2.0 34 1.0 35 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 9.090909090909092 54.54545454545454 18.181818181818183 18.181818181818183 2 27.27272727272727 9.090909090909092 45.45454545454545 18.181818181818183 3 9.090909090909092 36.36363636363637 18.181818181818183 36.36363636363637 4 9.090909090909092 9.090909090909092 36.36363636363637 45.45454545454545 5 9.090909090909092 54.54545454545454 36.36363636363637 0.0 6 27.27272727272727 36.36363636363637 18.181818181818183 18.181818181818183 7 18.181818181818183 36.36363636363637 27.27272727272727 18.181818181818183 8 9.090909090909092 63.63636363636363 9.090909090909092 18.181818181818183 9 18.181818181818183 27.27272727272727 18.181818181818183 36.36363636363637 10 9.090909090909092 27.27272727272727 45.45454545454545 18.181818181818183 11 27.27272727272727 45.45454545454545 9.090909090909092 18.181818181818183 12 36.36363636363637 18.181818181818183 45.45454545454545 0.0 13 27.27272727272727 9.090909090909092 36.36363636363637 27.27272727272727 14 27.27272727272727 36.36363636363637 18.181818181818183 18.181818181818183 15 9.090909090909092 27.27272727272727 45.45454545454545 18.181818181818183 16 27.27272727272727 36.36363636363637 27.27272727272727 9.090909090909092 17 0.0 27.27272727272727 45.45454545454545 27.27272727272727 18 0.0 63.63636363636363 27.27272727272727 9.090909090909092 19 27.27272727272727 18.181818181818183 54.54545454545454 0.0 20 27.27272727272727 45.45454545454545 9.090909090909092 18.181818181818183 21 9.090909090909092 36.36363636363637 45.45454545454545 9.090909090909092 22 9.090909090909092 18.181818181818183 54.54545454545454 18.181818181818183 23 0.0 45.45454545454545 36.36363636363637 18.181818181818183 24 0.0 36.36363636363637 27.27272727272727 36.36363636363637 25 18.181818181818183 27.27272727272727 45.45454545454545 9.090909090909092 26 9.090909090909092 27.27272727272727 36.36363636363637 27.27272727272727 27 18.181818181818183 36.36363636363637 27.27272727272727 18.181818181818183 28 27.27272727272727 18.181818181818183 27.27272727272727 27.27272727272727 29 18.181818181818183 18.181818181818183 27.27272727272727 36.36363636363637 30 0.0 45.45454545454545 27.27272727272727 27.27272727272727 31 18.181818181818183 45.45454545454545 27.27272727272727 9.090909090909092 32 27.27272727272727 45.45454545454545 18.181818181818183 9.090909090909092 33 27.27272727272727 18.181818181818183 45.45454545454545 9.090909090909092 34 9.090909090909092 27.27272727272727 63.63636363636363 0.0 35 27.27272727272727 27.27272727272727 27.27272727272727 18.181818181818183 36 9.090909090909092 27.27272727272727 45.45454545454545 18.181818181818183 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.5 25 1.0 26 2.0 27 3.0 28 3.0 29 2.0 30 1.0 31 1.0 32 0.5 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 2.5 39 5.0 40 2.5 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.5 47 1.0 48 1.0 49 0.5 50 0.0 51 0.0 52 0.0 53 0.0 54 0.0 55 0.0 56 0.0 57 0.0 58 0.0 59 0.0 60 0.0 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 36 11.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TCTCTAAATAGTTGTCCATAATTTGGTGCAAATATT 1 9.090909090909092 No Hit CTCAAAAACAAATGGTAATTCCACTAGCCCAGGTAT 1 9.090909090909092 No Hit ATCTATGCACCGCTTAAAAGTTTACTTGAAGCCTGT 1 9.090909090909092 No Hit AGTCTGCAGCAGCTAATATATTAATTCTTATAATAG 1 9.090909090909092 No Hit AGATAGAAGGCGTGATTTTGAATCATATGTAGGGGA 1 9.090909090909092 No Hit CAGCATGAATGTGCAATTGCGTAAACTCGTTTTTTC 1 9.090909090909092 No Hit TTCTTGACCTATGATTCAGCTAATTTAGTATTTTCC 1 9.090909090909092 No Hit ATACTATGCAAAAATGTAAAATCATCACACAAAACA 1 9.090909090909092 No Hit GTCCAATTCTGTGACATCTATCTTGACACTGATTGT 1 9.090909090909092 No Hit AGAGGCCATTTGCCTGCAGGAGACAAGACAAAGAAA 1 9.090909090909092 No Hit ACATACTAATATTACGATTATTCCTCATTCCGTTTT 1 9.090909090909092 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE