FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7425125
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG114820.15463712732108886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG15050.012.95918751
TAGGTAG9200.011.900595
CTAATAC20050.010.0236713
TAATACT26450.09.97983554
GTATAGA18200.09.8094911
TAGGCTG4650.09.676135
TAGTCAG9300.09.5149919
TAGACTG7650.09.4105125
GGTAGTC11450.09.3001977
GCGCACT5500.09.27205511
CTAAGCA10750.09.2081384
ACTGTGC7000.08.9989238
TATACTG22100.08.9580845
TCTAGAC10300.08.8823733
ACCGCGC3051.409353E-88.8513998
TTAGGTA16250.08.7681224
TCTAAGC10950.08.7660153
GTATTAA19500.08.6939021
CCGCGCA2957.7405275E-88.6430339
TATTAGG18850.08.5932512
Produced by FastQC (version 0.11.2)