FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1122975
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26270.23393218905140364No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCT550.00210262416.36244811
GTATTAG3000.015.5050871
GCCTCAC1251.13241185E-714.40023520
GGTTAGG851.6288464E-414.1222791
ACCTAGC750.001016766814.002129
GTGCTCC650.006389005613.8451479
GGTGCGA1201.1208631E-613.75144928
CGTGAGC1106.9142407E-613.63537212
CCGCGTT1004.2661086E-513.50142325
TGGCGAC1056.740019E-512.8590730
TAGGTAG2001.2732926E-1012.7490735
CTATGCA2950.012.7109399
GTGCGAC1302.7535934E-612.69421129
GGTCTAT954.1757128E-412.6357251
GCCCGCC2051.9281288E-1012.4381216
CACCTAG850.00261013312.35424328
CGCGTTC850.00261013312.35424326
CGGTGCG850.002611877412.35314322
GTCTTAT1006.431007E-412.0039381
GTATTAC1652.543038E-711.822061
Produced by FastQC (version 0.11.2)