FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2235736
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33270.1488100562857153No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCGCG650.00639513513.8439969
TAGGTAG3200.011.2479955
ACCGTGT1602.2687273E-611.2479958
ACTATGC4700.011.1682238
TCGCGCT950.00600357111.05165111
CCTAGAC1807.898434E-710.8316453
AGGACTG2104.5829438E-810.7123765
CCTAGCG1304.902993E-410.38810830
ATACCGT4350.010.3429846
GGACTGG1756.6316406E-610.2838826
CCTAATA6750.010.2206292
CTATGCA5050.010.0974369
GTATTAG5650.010.0896311
GTCTAGA2752.3665052E-99.8192871
GTATATA21100.09.6693351
GTATAGG4400.09.5465291
CTATACT6650.09.4719964
TCTATAC6350.09.4473473
CTATAGG2557.6506694E-89.4128251
ACTATAC7650.09.4102983
Produced by FastQC (version 0.11.2)