FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1526892
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29430.19274447701605613No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGCG559.588623E-519.089187
GGTAGTC2250.013.9987317
GTATTAG4000.013.5023151
CCTAGCG902.6489916E-413.33561930
CTGTGCG1151.0945274E-513.0422969
TAGGTAG2350.012.76485
TAGTCAG2204.9112714E-1112.2716169
ACCTCGC900.004010827711.6656098
ACCTAGC950.00599194611.05416429
ACTTCGC950.00600217811.051638
CTAGGAC1100.001434233510.9081033
GTATAGA4600.010.7627141
TTATACA5800.010.6024884
GTAGTCA3400.010.5872768
ATTATAC6400.010.5459193
AGTCAGA2853.8198777E-1110.52570710
CGCGCTT1000.00878092910.49973712
AGTGCCG1000.00878092910.49973721
TGCACCG1000.00878519310.4990495
GTGTGCA2156.457776E-810.4641689
Produced by FastQC (version 0.11.2)