FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1041615
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGGG601.8658303E-417.49955215
CGGTCGG851.6335676E-414.11728713
CTGTGTG650.00639066513.8444699
GGATAGG850.002608718412.3549981
CCGTCGC850.002614361212.3514399
CTGTGCG850.002614361212.3514399
CTAATAC3050.012.2929963
GGTCGGG1006.449459E-411.99969314
TAGGTAG1501.0407985E-611.9979655
GGTTATA1406.3005973E-611.7876761
CTATACT2553.6379788E-1211.7627114
CTTAAGG900.004011733511.6646883
GACAGTC1303.8493454E-511.5365047
TAGACAG1303.8493454E-511.5365045
ATCACAC1703.7539758E-711.47194729
GACAGCG1059.723259E-411.4266337
CCTACAC1202.3473987E-411.2480923
TTAGGTA2155.4133125E-911.1608974
TCTATAC2701.2732926E-1111.1092263
GTCTTAT1901.3199315E-711.0544711
Produced by FastQC (version 0.11.2)