FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1658359
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24230.1461082913892589No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG3600.014.16715051
GTCCTAG1004.2713684E-513.5004611
TAGGTAG2700.012.7762885
GTCTTAA1857.021299E-912.1625791
CCTAATA4100.012.0717632
TATTATA7250.011.7917292
TATACTG5500.011.7259055
ACACTGT2206.2937033E-1011.5899076
CTTACAC2206.2937033E-1011.5895573
CCTAGCG1303.8409904E-511.53990130
TACACAG3150.011.4272395
GTATAGA4650.011.2907091
CTGATCG1355.6556957E-511.1101519
GTATAAC3650.011.096271
CTGCGAG950.006002363311.0516769
GTATATA8500.010.941551
CTATGCA3450.010.8686269
TCTATAC4050.010.7394893
CTCTTAA3650.010.6852971
GTATTAT7600.010.6582591
Produced by FastQC (version 0.11.2)