FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1280600
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19840.15492737779166016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCGG1154.0594387E-815.6574331
ACCCGTC750.001018778313.9986897
ATGCCTA800.001657756713.123775
TAGACTG1056.7568595E-512.8559385
CTCCGCA1302.7619808E-612.6911199
CCGACTA954.1899292E-412.63088814
CTCGGAG1201.6952416E-512.4988283
TCGGTCG1101.043991E-412.2715779
ATAGGGT1601.7149796E-712.1863583
CGGCAGG1151.5788976E-411.73894722
AGGCCGC900.00401031811.6655736
TATCCTG3100.011.61181455
GCGAGGC1202.3406013E-411.25202130
TAGACAG2402.2191671E-1011.2489455
CGGTCGA1202.3465458E-411.24894510
ACTGCCC1100.001433997410.9080698
CTAATAC2751.8189894E-1110.9080693
TTAGACA2351.875378E-910.8500474
GTATAGA3600.010.8369731
TGCGAGG1253.4162338E-410.8019429
Produced by FastQC (version 0.11.2)