FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3475835
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG65890.18956596040951312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG3900.011.1528145
GTATTAG8750.010.8019531
TATACGG10350.010.2889035
TACGGTG7650.010.1951357
ATACGGT9400.010.0522616
ACGGTGT7650.09.9990758
GTGCGAC2454.128924E-89.79712629
CTAATAC11550.09.7394983
CCGTCCA1851.2835739E-59.7288289
GTATAGA10250.09.6602841
TTAGGTA7500.09.5991124
CGGTGTT6600.09.5445719
TAGACTG3552.7284841E-119.2949145
GTATAAT15550.09.2621251
CTAAGAC5200.09.2300473
CGCGCAT1300.00572942579.22991510
GTATTAT17100.09.2121921
GTCTAAG4750.09.1595511
GTATATA28050.09.1460391
ACTATGC9050.09.1151788
Produced by FastQC (version 0.11.2)